Journal: BMC Cancer

Article Title: Identification of a Novel CD8 + T cell exhaustion-related gene signature for predicting survival in hepatocellular carcinoma

doi: 10.1186/s12885-023-11648-x

Figure Lengend Snippet: Expression of STAM, ANXA5 and MAD2L2 in HCC cell lines. A The expressions of STAM, ANXA5 and MAD2L2 in normal hepatocytes and hepatoma cell lines were detected using Western blotting. Knocking down STAM, ANXA5 and MAD2L2 inhibited the proliferation and migration of HCC cells. B The knockdown efficiency of STAM, ANXA5 and MAD2L2 was detected using Western Blotting. C Plate cloning experiment showed that the number of cloned cell clusters formed by hepatocellular carcinoma cells after knockdown of STAM, ANXA5 and MAD2L2 was significantly reduced; D The results of CCK8 experiment showed that inhibiting the expression of STAM, ANXA5 and MAD2L2 decreased the proliferative ability of HCC cells; E Scratch assay showed that knockdown of STAM, ANXA5 and MAD2L2 inhibited the migration of hepatocellular carcinoma cells; F Transwell experiment showed that the migration ability of hepatocellular carcinoma cells was weakened after inhibiting the expression of STAM, ANXA5 and MAD2L2 .* P < 0.05, ** P < 0.01, *** P < 0.001, there was no significant difference in ns

Article Snippet: Anti- MAD2L2 (BM5428) and anti- STAM (A00864-1) antibodies were purchased from BosterBio (Pleasanton, CA, USA), while Anti- TBL1XR1 (MBS850368), Anti- ANXA5 (MBS474163), Anti- FKBP1A (MBS9404059) and Anti- PPM1G (MBS626576) antibodies were purchased from MyBioSource (San Diego, CA, USA).

Techniques: Expressing, Western Blot, Migration, Knockdown, Cloning, Clone Assay, Wound Healing Assay

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 8. Effects of TFE from Folium isatidis on the genes expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d) and p65 (e) in lungs tissues of LPS induced ALI mice ( # compared with the control, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing, Control

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 9. Effects of TFE from Folium isatidis on the proteins expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d), p-I 𝜅B (e), p65 (f) and p-p65 (g) in lungs tissues of LPS induced ALI mice ( # compared with the cntrol, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 10. Regulation of TFE from Folium isatidis on TLR4-TRIF-NF- 𝜅B signaling pathway in LPS induced ALI mice.

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway

doi: 10.1186/s12974-019-1457-9

Figure Lengend Snippet: Expression of GPR40 in microglia ( a – c ), astrocytes ( d – f ), and neurons ( g – i ) in the spinal dorsal horn of neuropathic rats. Frozen sections of the spinal lumbar enlargements were obtained approximately 2 weeks after spinal nerve ligation. Immunofluorescence was double stained with GPR40/Iba-1, GPR40/GFAP, and GPR40/NeuN, and photomicrographs were taken from the spinal cord section ( a , d , g ; 500 μm) and amplified dorsal horn laminae I–V ( b , c , e , f , h , i ; 50 μm). Arrows indicate double immunostaining of GPR40 with each cellular biomarker. Double immunolabeled surface areas of GPR40/Iba-1 ( j ), GPR40/GFAP ( k ), and GPR40/NeuN ( l ) from the spinal dorsal horn laminae I–V indicated in white lines were quantified using the ImageJ program. Data are presented as means ± SEM ( N = 5–6 per group). * P < 0.05 vs. contralateral side; analyzed by unpaired and two-tailed Student’s t test

Article Snippet: Iba-1 , Merck Millipore, Darmstadt, Germany , 1:300 , 1:100.

Techniques: Expressing, Ligation, Immunofluorescence, Staining, Amplification, Double Immunostaining, Biomarker Assay, Immunolabeling, Two Tailed Test

Journal: Journal of Neuroinflammation

Article Title: Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway

doi: 10.1186/s12974-019-1457-9

Figure Lengend Snippet: Effect of intrathecal GW9508 on spinal IL-10 expression on spinal microglia ( a – f ), astrocytes ( g – l ), and neurons ( m – r ) in neuropathic rats induced by spinal nerve ligation. Frozen sections of the spinal lumbar enlargements were obtained 1 h after intrathecal saline (10 μl) or GW9508 (30 μg) treatment. Immunofluorescence was double stained with IL-10/Iba-1, IL-10/GFAP, and IL-10/NeuN, and photomicrographs were taken from the entire spinal cord section ( a , d , g , j , m , p ; 500 μm) and amplified dorsal horn laminae I–V ( b , c , e , f , h , i , k , l , n , o , q , r ; 50 μm). Arrows indicate double immunostaining of IL-10 and each cellular biomarker. Double immunolabeled surface areas of IL-10/Iba-1 ( s ), IL-10/GFAP ( t ), and IL-10/NeuN ( u ) from the spinal dorsal horn laminae I-V indicated in white lines were quantified using the ImageJ program. Data are presented as means ± SEM ( N = 5~7 per group). * P < 0.05 vs. saline group; analyzed by unpaired and two-tailed Student’s t test

Article Snippet: Iba-1 , Merck Millipore, Darmstadt, Germany , 1:300 , 1:100.

Techniques: Expressing, Ligation, Immunofluorescence, Staining, Amplification, Double Immunostaining, Biomarker Assay, Immunolabeling, Two Tailed Test

Journal: Journal of Neuroinflammation

Article Title: Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway

doi: 10.1186/s12974-019-1457-9

Figure Lengend Snippet: Effect of GW9508 given intrathecally on spinal β-endorphin expression on spinal microglia ( a – f ), astrocytes ( g – l ), and neurons ( m – r ) in neuropathic rats induced by spinal nerve ligation. Frozen sections of the spinal lumbar enlargements were obtained 1 h after intrathecal saline (10 μl) or GW9508 (30 μg) treatment. Immunofluorescence was double stained with β-endorphin/Iba-1, β-endorphin/GFAP, and β-endorphin/NeuN, and photomicrographs were taken from the entire spinal cord section ( a , d , g , j , m , p : 500 μm) and amplified dorsal horn laminae I–V ( b , c , e , f , h , i , k , l , n , o , q , r : 50 μm). Arrows indicate double immunostaining of β-endorphin with each cellular biomarker. Double immunolabeled surface areas of β-endorphin/Iba-1 ( s ), β-endorphin/GFAP ( t ), and β-endorphin/NeuN ( u ) from the spinal dorsal horn laminae I-V indicated in white lines were quantified using the ImageJ program. Data are presented as means ± SEM ( N = 5~7 per group). * P < 0.05 vs. saline group; analyzed by unpaired and two-tailed Student’s t test

Article Snippet: Iba-1 , Merck Millipore, Darmstadt, Germany , 1:300 , 1:100.

Techniques: Expressing, Ligation, Immunofluorescence, Staining, Amplification, Double Immunostaining, Biomarker Assay, Immunolabeling, Two Tailed Test

Journal: Journal of Neuroinflammation

Article Title: Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway

doi: 10.1186/s12974-019-1457-9

Figure Lengend Snippet: Expression of GPR40 ( a – c ) and stimulatory effects of GW9508 on mRNA expression of IL-10, the β-endorphin precursor proopiomelanocortin (POMC) and dynorphin A precursor prodynorphin (PDYN) ( d – g ), and secretion of IL-10 and β-endorphin ( h , i ) in cultured primary microglia, astrocytes, and neurons originated from the spinal cords of 1-day-old neonatal rats. For the immunostaining study, immunofluorescence was double stained with GPR40/Iba-1, GPR40/GFAP, and GPR40/NeuN. For the stimulatory study, cultured cells and cultural medium were collected 2 h after GW9508 incubation, and the mRNA expression of IL-10, POMC, and PDYN and the peptide concentrations of IL-10 and β-endorphin were measured using quantitative real-time PCR and commercial ELISA kits, respectively. Data are presented as means ± SEM ( N = 4~7 per group). * P < 0.05 vs. control group; analyzed by unpaired and two-tailed Student’s t test

Article Snippet: Iba-1 , Merck Millipore, Darmstadt, Germany , 1:300 , 1:100.

Techniques: Expressing, Cell Culture, Immunostaining, Immunofluorescence, Staining, Incubation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Two Tailed Test